Circadian Clock Associated 1 (CCA1) is a gene that is central to the

circadian oscillator A circadian clock, or circadian oscillator, is a biochemical oscillator that cycles with a stable phase and is synchronized with solar time. Such a clock's ''in vivo'' period is necessarily almost exactly 24 hours (the earth's current solar day ...

angiosperms Flowering plants are plants that bear flowers and fruits, and form the clade Angiospermae (), commonly called angiosperms. The term "angiosperm" is derived from the Greek words ('container, vessel') and ('seed'), and refers to those plants th ...

. It was first identified in ''

Arabidopsis thaliana ''Arabidopsis thaliana'', the thale cress, mouse-ear cress or arabidopsis, is a small flowering plant native to Eurasia and Africa. ''A. thaliana'' is considered a weed; it is found along the shoulders of roads and in disturbed land. A winter a ...

'' in 1993. CCA1 interacts with LHY and TOC1 to form the core of the oscillator system. CCA1 expression peaks at dawn. Loss of CCA1 function leads to a shortened period in the expression of many other genes.

Discovery

CCA1 was first identified in ''Arabidopsis thaliana'' by Elaine M. Tobin’s lab in

UCLA The University of California, Los Angeles (UCLA) is a public land-grant research university in Los Angeles, California. UCLA's academic roots were established in 1881 as a teachers college then known as the southern branch of the California St ...

in 1993. Tobin’s lab was studying promoter fragments that contribute to light regulation of light-harvesting Chlorophyll A/B Binding Protein (LHCB), and noticed DNA-binding activity that had affinity for a specific light-responsive fragment of the LHCB promoter. This DNA-binding activity was designated as CA-1 because the binding is mostly to

cytosine Cytosine () ( symbol C or Cyt) is one of the four nucleobases found in DNA and RNA, along with adenine, guanine, and thymine (uracil in RNA). It is a pyrimidine derivative, with a heterocyclic aromatic ring and two substituents attached (an am ...

and

adenine Adenine () ( symbol A or Ade) is a nucleobase (a purine derivative). It is one of the four nucleobases in the nucleic acid of DNA that are represented by the letters G–C–A–T. The three others are guanine, cytosine and thymine. Its derivati ...

-rich sequences. They found that this binding activity is necessary for

phytochrome Phytochromes are a class of photoreceptor in plants, bacteria and fungi used to detect light. They are sensitive to light in the red and far-red region of the visible spectrum and can be classed as either Type I, which are activated by far-re ...

response. They later found the gene responsible for this activity by screening the ''Arabidopsis'' expression library. CCA1 referred to the CA-1 clone, and now designated the gene responsible for this DNA binding activity. The evolution of circadian clocks in land plants is not understood, because circadian rhythms have received little attention in plants other than angiosperms.

Function

CCA1 is part of a negative autoregulatory feedback loop that is coordinated with the daily environmental changes. Repressed in the dark by other proteins, CCA1’s expression is activated when light is sensed by the phytochrome in the plant. After translation, the CCA1 protein needs to be phosphorylated by

Casein Kinase 2 Casein kinase 2 ()(CK2/CSNK2) is a serine/threonine-selective protein kinase that has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm, and other cellular processes. De-regulation of CK2 has been linked to tumor ...

(CK2). This

phosphorylation In chemistry, phosphorylation is the attachment of a phosphate group to a molecule or an ion. This process and its inverse, dephosphorylation, are common in biology and could be driven by natural selection. Text was copied from this source, wh ...

is necessary for the protein to form a

homodimer In biochemistry, a protein dimer is a macromolecular complex formed by two protein monomers, or single proteins, which are usually non-covalently bound. Many macromolecules, such as proteins or nucleic acids, form dimers. The word ''dimer'' ha ...

and to bind to its target promoters.

Hyperphosphorylation Hyperphosphorylation occurs when a biochemical with multiple phosphorylation sites is fully saturated. Hyperphosphorylation is one of the signaling mechanisms used by the cell to regulate mitosis. When these mechanisms fail, developmental problems ...

, due to the overexpression of CK2, will lead to altered circadian rhythms in the mutants where CCA1 showed shorter mRNA circadian oscillation than in wild-type plants. CK2 overexpression is significant in demonstrating CCA1 is part of the clock. The protein motif CCA1 uses to bind to its target DNA sequences is its Myb-like domain. CCA1 only has one Myb domain, whereas other plant and mammalian proteins could have multiple Myb domains. The presence of only one Myb domain in CCA1 shows its importance influence in the circadian clock.

LUX The lux (symbol: lx) is the unit of illuminance, or luminous flux per unit area, in the International System of Units (SI). It is equal to one lumen per square metre. In photometry, this is used as a measure of the intensity, as perceived by the ...

is also an important Myb transcription factor that is necessary for CCA/LHY transcription. This can also help account for problems in the

repressilator The repressilator is a genetic regulatory network consisting of at least one feedback loop with at least three genes, each expressing a protein that represses the next gene in the loop. In biological research, repressilators have been used to build ...

model described below. CCA1 is also unusual in that it has the ability to bind to asymmetric DNA sequences. CCA1 acts to suppress the expression of the DNA sequence it binds to. The stability of CCA1 protein is not affected by light or dark. It is regulated by its

proteasome Proteasomes are protein complexes which degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds. Enzymes that help such reactions are called proteases. Proteasomes are part of a major mechanism by w ...

. Inhibiting proteasome function leads to a circadian rhythm with a longer period.

CCA1 and the ''Arabidopsis'' circadian oscillator

The Arabidopsis central oscillator contains several proteins that reciprocally repress genes encoding each other to achieve a negative feedback loop necessary to generate circadian rhythms controlling many clock outputs. CCA1 is a key component of this oscillator. Light induces its transcription, and mRNA levels peak at dawn along with Late Elongated Hypocotyl (LHY). CCA1 and LHY associate to inhibit transcription of the Evening Complex (EC) proteins: ELF4, ELF3 and LUX, which suppresses their accumulation until dusk when LHY and CCA1 protein levels are at their lowest. The EC inhibits transcription PRR9 and TOC1 at night. These, along with the remaining PRR proteins PRR7 and PRR5 are involved in suppressing CCA1 and LHY levels, which increase during the night. CCA1 is further involved in maintaining this loop by inhibiting its own expression.

Homologs

Paralogs

LHY (late elongated hypocotyl) also has a Myb domain and functions early in the morning. Both LHY and CCA1 have similar patterns of expression, which could be induced by light. Single loss of function mutants in both genes result in seemingly identical phenotypes. But LHY cannot fully rescue the rhythm when CCA1 is absent, indicating that they may only be partially functionally redundant. Under constant light conditions, cca1 and lhy double loss of function mutants fail to maintain rhythms in clock controlled RNAs.

Orthologs

The circadian oscillator in rice is similar to the ''Arabidopsis'' model, and researchers have used this model as a blueprint for understand the rice oscillator. OsLHY in rice serves a similar function as CCA1/LHY and is thus an ortholog of the gene in rice. OsPRR1 in rice is also an ortholog of TOC1. PpCCA1a and PpCCA1b are orthologs of CCA1 and LHy in the moss Physcomitrella patens. They show rhythms with a period of 1 day like their angiosperm homologs in 24-hour light-dark cycles or constant darkness. However these genes show arrhythmicity in constant light conditions, in contrast to CCA1:LHY.

Mutants

Mutants such as cca1-1 plants, which lack CCA1 protein, show short period phenotypes for the expression of several genes when assayed under constant light conditions. They also have a period 3 hours shorter than that of the wild-type plant, which demonstrates that expression of LHY, its homolog, cannot fully compensate for the loss of the function of CCA1. Plants that have lost function of LHY and CCA1 (lhy;cca1) lost the ability to stably maintain circadian rhythm and other output phenomena. In one study, lhy;cca1 show photoperiod- insensitive early flowering under long- day (16 hours of light/ 8 hours of dark) conditions and short day (8 hours of light, 16 hours of dark conditions), and arrhythmicity under constant light conditions. However they retain some circadian function in light/dark cycles, showing that ''Arabidopsis'' circadian clock is not completely dependent on CCA1 and LHY activity. Plants with non-functioning LHY and CCA1 show a wavy leaf phenotype in constant light conditions. Mutants also have increased vascular pattern complexity in their leaves, with more areoles, branch points and free ends than wild-type ''Arabidopsis''.

References

{{Reflist Plant genes